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In vitro plant regeneration from hypocotyl explants of Rheum webbianum Royle

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In vitro plant regeneration from hypocotyl explants of Rheum webbianum Royle


Sabreena Rashid | Zahoor A Kaloo | Seema Singh | Iram Bashir

https://doi.org/10.31142/ijtsrd9558



Sabreena Rashid | Zahoor A Kaloo | Seema Singh | Iram Bashir "In vitro plant regeneration from hypocotyl explants of Rheum webbianum Royle" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2, February 2018, pp.1135-1139, URL: https://www.ijtsrd.com/papers/ijtsrd9558.pdf

Rheum webbianum commonly known as “rhubarb” is a reputed medicinal plant belonging to family Polygonaceae. An attempt has been made to carry out the in vitro regeneration of this important medicinal plant species using hypocotyl segments as explants. Direct organogenesis occurred when the hypocotyl segments from the in vitro germinated seedlings were inoculated on MS medium containing different growth regulators at various concentrations and combinations. Cytokinins like BAP and Kn were found to be effective in shoot regeneration both individually as well as in combination with auxins like IAA, IBA and 2,4-D MS medium supplemented with BAP at a concentration of 2mg/l in combination with IAA at a concentration of 3mg/l was found to be the best medium for shoot regeneration. For rooting of regenerated shoots half strength MS medium supplemented with 0.5mg/l IAA was found to be most effective.

Organogenesis, hypocotyl, cytokinins


IJTSRD9558
Volume-2 | Issue-2, February 2018
1135-1139
IJTSRD | www.ijtsrd.com | E-ISSN 2456-6470
Copyright © 2019 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0)

International Journal of Trend in Scientific Research and Development - IJTSRD having online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International Journal which provides rapid publication of your research articles and aims to promote the theory and practice along with knowledge sharing between researchers, developers, engineers, students, and practitioners working in and around the world in many areas like Sciences, Technology, Innovation, Engineering, Agriculture, Management and many more and it is recommended by all Universities, review articles and short communications in all subjects. IJTSRD running an International Journal who are proving quality publication of peer reviewed and refereed international journals from diverse fields that emphasizes new research, development and their applications. IJTSRD provides an online access to exchange your research work, technical notes & surveying results among professionals throughout the world in e-journals. IJTSRD is a fastest growing and dynamic professional organization. The aim of this organization is to provide access not only to world class research resources, but through its professionals aim to bring in a significant transformation in the real of open access journals and online publishing.

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